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Ancient DNA analysis reveals how the rise and fall of the Roman Empire shifted populations in the Balkans
Despite the Roman Empire’s extensive military and cultural influence on the nearby Balkan peninsula, a DNA analysis of individuals who lived in the region between 1 and 1000 CE found no genetic evidence of Iron Age Italian ancestry. Instead, a study published December 7 in the journal Cell revealed successive waves of migrations from Western Anatolia, central and northern Europe, and the Pontic-Kazakh Steppe during the Empire’s reign.
“We found this genetic signal of Slavic migration all across the Balkans,” says senior author and paleogenomicist Carles Lalueza-Fox of the Institute of Evolutionary Biology (IBE:CSIC-Universitat Pompeu Fabra) and Museu de Ciències Naturals de Barcelona. “This could have important social and political implications given that the Balkans has had a long history of conflict associated with their perceived identities.”
Most ancient DNA studies focus on pre-history — before the written record — but ancient DNA methods can also provide insight into more recent historical periods, especially when used in combination with historical and archeological information.
“Ancient DNA can give a lot of insight into historical periods, especially for regions where historical sources are scarce or when we don’t know whether sources are biased or not,” says first author and population geneticist Iñigo Olalde of the University of the Basque Country (UPV/EHU). “For example, most historical sources from the Balkans are written from the side of the Romans because the Slavic people didn’t write at that time.”
Previous studies have investigated the ancestry of people who lived in Italy and England during and after the fall of the Roman Empire, but little is known about demography and ancestry of the Balkans during this time. “This region was one of the distant frontiers of the Roman Empire, which makes it interesting to study because this is clearly a place where you would expect people to come in contact with people from outside the Empire, so you can test things such as globalization,” says Olalde.
To explore the population history of the Balkans and examine the influence of the rise and fall of the Roman empire, the researchers extracted DNA from 136 ancient individuals excavated from 20 different sites across the Balkans — defined as the region bounded by the Adriatic, Central Mediterranean, and Aegean Seas and the Middle and Lower Danube and Sava Rivers. These sites included large Roman cities, military fortresses, and small rural towns. The team focused on three periods: during the expansion and height of the Roman empire (1-250 CE), during the late Imperial period (circa 250-550 CE), and following the Western Empire’s collapse (550-1000 CE).
To provide cultural and historical context for the genetic data, the team collaborated with local archeologists and historians. For each grave, they documented burial type, as well as any objects buried alongside the individuals, such as coins, jewelry, pottery, tools, and weapons. The researchers also used radiocarbon dating to verify the age of 38 of the ancient individuals, which generated isotopic data that provide a window into those individuals’ diets.
The researchers were surprised to find no evidence of Italian Iron Age ancestry in the Balkan populations during the height of the Roman Empire. Instead, they showed that there was an influx of people from Western Anatolia, another part of the Roman Empire, during that period. They also found evidence of individual migrations into the Balkans from both within and outside the Roman Empire. Notably, a 16-year-old male who was excavated from a necropolis in a large Roman city was of 100% East African ancestry. The individual was buried with an oil lamp depicting Jupiter-related eagle iconography, but isotopic analysis of his teeth indicated that he had consumed marine protein sources during his childhood and therefore had likely grown up in a distant location.
“This was the only full Eastern African individual that we analyzed, and he was also a clear outlier with respect to the diet compared to the rest of the individuals buried in the same necropolis, which tells us that this individual clearly grew up outside the borders of the Roman Empire,” says Lalueza-Fox.
During the late Imperial period, between 250 and 550 CE, the researchers detected migrants with mixed ancestry from Northern Europe and the Pontic-Kazakh steppe. “We found that those two ancestries — central/northern European and Sarmatian-Scythian — tended to come together, which suggests that these are likely to have been multi-ethnic confederations of moving people,” says senior author and population geneticist David Reich of Harvard University.
However, these sources of ancestry disappeared after 700 CE. From 600 CE, shortly after the fall of the Western Roman Empire, there was a major influx of individuals from Eastern Europe. After 700 CE, individuals in the Balkans had very similar ancestral composition to present-day groups in the region, suggesting that these migrations resulted in the last large demographic shift in the area. These migrations coincide with recorded Slavic migrations, but the DNA analysis provides insight into the scale of these migrations that is impossible to glean from historical resources.
“There have been debates about how impactful these migrations were and to what extent the spread of Slavic language was largely through cultural influences or movements of people, but our study shows that these migrations had a profound demographic effect,” says Reich. “More than half of the ancestry of most peoples in the Balkans today comes from the Slavic migrations, with around a third Slavic ancestry even in countries like Greece where no Slavic languages are spoken today.”
The team are already planning what they call “version two” of the study, which will take advantage of improvements in ancient DNA technologies. “We are now able to sequence hundreds of individuals from the same site, so we can go to another level of resolution and start to understand more about the social interactions and kinship between the different individuals,” says Olalde.
This research was supported by the Spanish Ministry of Science of Innovation, ‘la Caixa” Foundation, the Natural Sciences and Engineering Research Council of Canada, the Ministry of Science and Education of the Republic of Croatia, the National Institutes of Health, the John Templeton Foundation, the Allen Discovery Center, the Paul G. Allen Family Foundation, and the Howard Hughes Medical Institute.
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Early dark energy could resolve cosmology’s two biggest puzzles
A new study by MIT physicists proposes that a mysterious force known as early dark energy could solve two of the biggest puzzles in cosmology and fill in some major gaps in our understanding of how the early universe evolved.
Now, the MIT team has found that both puzzles could be resolved if the early universe had one extra, fleeting ingredient: early dark energy. Dark energy is an unknown form of energy that physicists suspect is driving the expansion of the universe today. Early dark energy is a similar, hypothetical phenomenon that may have made only a brief appearance, influencing the expansion of the universe in its first moments before disappearing entirely.
Some physicists have suspected that early dark energy could be the key to solving the Hubble tension, as the mysterious force could accelerate the early expansion of the universe by an amount that would resolve the measurement mismatch.
The MIT researchers have now found that early dark energy could also explain the baffling number of bright galaxies that astronomers have observed in the early universe. In their new study, reported in the Monthly Notices of the Royal Astronomical Society, the team modeled the formation of galaxies in the universe’s first few hundred million years. When they incorporated a dark energy component only in that earliest sliver of time, they found the number of galaxies that arose from the primordial environment bloomed to fit astronomers’ observations.
“You have these two looming open-ended puzzles,” says study co-author Rohan Naidu, a postdoc in MIT’s Kavli Institute for Astrophysics and Space Research. “We find that in fact, early dark energy is a very elegant and sparse solution to two of the most pressing problems in cosmology.”
The study’s co-authors include lead author and Kavli postdoc Xuejian (Jacob) Shen, and MIT professor of physics Mark Vogelsberger, along with Michael Boylan-Kolchin at the University of Texas at Austin, and Sandro Tacchella at the University of Cambridge.
Big city lights
Based on standard cosmological and galaxy formation models, the universe should have taken its time spinning up the first galaxies. It would have taken billions of years for primordial gas to coalesce into galaxies as large and bright as the Milky Way.
But in 2023, NASA’s James Webb Space Telescope (JWST) made a startling observation. With an ability to peer farther back in time than any observatory to date, the telescope uncovered a surprising number of bright galaxies as large as the modern Milky Way within the first 500 million years, when the universe was just 3 percent of its current age.
“The bright galaxies that JWST saw would be like seeing a clustering of lights around big cities, whereas theory predicts something like the light around more rural settings like Yellowstone National Park,” Shen says. “And we don’t expect that clustering of light so early on.”
For physicists, the observations imply that there is either something fundamentally wrong with the physics underlying the models or a missing ingredient in the early universe that scientists have not accounted for. The MIT team explored the possibility of the latter, and whether the missing ingredient might be early dark energy.
Physicists have proposed that early dark energy is a sort of antigravitational force that is turned on only at very early times. This force would counteract gravity’s inward pull and accelerate the early expansion of the universe, in a way that would resolve the mismatch in measurements. Early dark energy, therefore, is considered the most likely solution to the Hubble tension.
Galaxy skeleton
The MIT team explored whether early dark energy could also be the key to explaining the unexpected population of large, bright galaxies detected by JWST. In their new study, the physicists considered how early dark energy might affect the early structure of the universe that gave rise to the first galaxies. They focused on the formation of dark matter halos — regions of space where gravity happens to be stronger, and where matter begins to accumulate.
“We believe that dark matter halos are the invisible skeleton of the universe,” Shen explains. “Dark matter structures form first, and then galaxies form within these structures. So, we expect the number of bright galaxies should be proportional to the number of big dark matter halos.”
The team developed an empirical framework for early galaxy formation, which predicts the number, luminosity, and size of galaxies that should form in the early universe, given some measures of “cosmological parameters.” Cosmological parameters are the basic ingredients, or mathematical terms, that describe the evolution of the universe.
Physicists have determined that there are at least six main cosmological parameters, one of which is the Hubble constant — a term that describes the universe’s rate of expansion. Other parameters describe density fluctuations in the primordial soup, immediately after the Big Bang, from which dark matter halos eventually form.
The MIT team reasoned that if early dark energy affects the universe’s early expansion rate, in a way that resolves the Hubble tension, then it could affect the balance of the other cosmological parameters, in a way that might increase the number of bright galaxies that appear at early times. To test their theory, they incorporated a model of early dark energy (the same one that happens to resolve the Hubble tension) into an empirical galaxy formation framework to see how the earliest dark matter structures evolve and give rise to the first galaxies.
“What we show is, the skeletal structure of the early universe is altered in a subtle way where the amplitude of fluctuations goes up, and you get bigger halos, and brighter galaxies that are in place at earlier times, more so than in our more vanilla models,” Naidu says. “It means things were more abundant, and more clustered in the early universe.”
“A priori, I would not have expected the abundance of JWST’s early bright galaxies to have anything to do with early dark energy, but their observation that EDE pushes cosmological parameters in a direction that boosts the early-galaxy abundance is interesting,” says Marc Kamionkowski, professor of theoretical physics at Johns Hopkins University, who was not involved with the study. “I think more work will need to be done to establish a link between early galaxies and EDE, but regardless of how things turn out, it’s a clever — and hopefully ultimately fruitful — thing to try.”
“We demonstrated the potential of early dark energy as a unified solution to the two major issues faced by cosmology. This might be an evidence for its existence if the observational findings of JWST get further consolidated,” Vogelsberger concludes. “In the future, we can incorporate this into large cosmological simulations to see what detailed predictions we get.”
This research was supported, in part, by NASA and the National Science Foundation.
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Plant-derived secondary organic aerosols can act as mediators of plant-plant interactions
A new study published in Science reveals that plant-derived secondary organic aerosols (SOAs) can act as mediators of plant-plant interactions. This research was conducted through the cooperation of chemical ecologists, plant ecophysiologists and atmospheric physicists at the University of Eastern Finland.
The study showed that Scots pine seedlings, when damaged by large pine weevils, release VOCs that activate defences in nearby plants of the same species. Interestingly, the biological activity persisted after VOCs were oxidized to form SOAs. The results indicated that the elemental composition and quantity of SOAs likely determines their biological functions.
“A key novelty of the study is the finding that plants adopt subtly different defence strategies when receiving signals as VOCs or as SOAs, yet they exhibit similar degrees of resistance to herbivore feeding,” said Professor James Blande, head of the Environmental Ecology Research Group. This observation opens up the possibility that plants have sophisticated sensing systems that enable them to tailor their defences to information derived from different types of chemical cue.
“Considering the formation rate of SOAs from their precursor VOCs, their longer lifetime compared to VOCs, and the atmospheric air mass transport, we expect that the ecologically effective distance for interactions mediated by SOAs is longer than that for plant interactions mediated by VOCs,” said Professor Annele Virtanen, head of the Aerosol Physics Research Group. This could be interpreted as plants being able to detect cues representing close versus distant threats from herbivores.
The study is expected to open up a whole new complex research area to environmental ecologists and their collaborators, which could lead to new insights on the chemical cues structuring interactions between plants.
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Folded or cut, this lithium-sulfur battery keeps going
Most rechargeable batteries that power portable devices, such as toys, handheld vacuums and e-bikes, use lithium-ion technology. But these batteries can have short lifetimes and may catch fire when damaged. To address stability and safety issues, researchers reporting in ACS Energy Letters have designed a lithium-sulfur (Li-S) battery that features an improved iron sulfide cathode. One prototype remains highly stable over 300 charge-discharge cycles, and another provides power even after being folded or cut.
The team coated iron sulfide cathodes in different polymers and found in initial electrochemical performance tests that polyacrylic acid (PAA) performed best, retaining the electrode’s discharge capacity after 300 charge-discharge cycles. Next, the researchers incorporated a PAA-coated iron sulfide cathode into a prototype battery design, which also included a carbonate-based electrolyte, a lithium metal foil as an ion source, and a graphite-based anode. They produced and then tested both pouch cell and coin cell battery prototypes.
After more than 100 charge-discharge cycles, Wang and colleagues observed no substantial capacity decay in the pouch cell. Additional experiments showed that the pouch cell still worked after being folded and cut in half. The coin cell retained 72% of its capacity after 300 charge-discharge cycles. They next applied the polymer coating to cathodes made from other metals, creating lithium-molybdenum and lithium-vanadium batteries. These cells also had stable capacity over 300 charge-discharge cycles. Overall, the results indicate that coated cathodes could produce not only safer Li-S batteries with long lifespans, but also efficient batteries with other metal sulfides, according to Wang’s team.
The authors acknowledge funding from the National Natural Science Foundation of China; the Natural Science Foundation of Sichuan, China; and the Beijing National Laboratory for Condensed Matter Physics.
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